Troy C M, Brown K, Greene L A, Shelanski M L
Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Neuroscience. 1990;36(1):217-37. doi: 10.1016/0306-4522(90)90364-a.
The expression of peripherin, a type III neuron-specific intermediate filament protein, and the middle neurofilament subunit were studied in the mouse embryo using immunofluorescence staining. The earliest staining for both proteins is seen at embryonic day 9 in the myelencephalon, initially as fiber staining followed by cell body staining in the developing facial and acoustic nuclei. As the embryo develops, there is rostral as well as caudal extension of peripherin and staining is seen in the trigeminal ganglia, nerve fibers and in the enteric nervous system. As the spinal cord forms there is anti-peripherin staining in developing motoneurons of the anterior horns while little cell body staining is seen for the middle neurofilament subunit. Both antibodies stain the developing dorsal root and its entry zone, but peripherin is found in the secondary sensory and commissural fibers while the middle neurofilament subunit is not. While both proteins are found in the neurons of the dorsal root ganglia, their distribution varies. The larger peripheral cells of the ganglia contain both proteins while the smaller more central cells, constituting over 60% of the cells in the ganglia, contain only peripherin. A similar picture is found in the sympathetic ganglia where there are cells which contain peripherin. middle neurofilament subunit or both, but where the majority of the neurons have only peripherin in their cell bodies. Peripherin is not found in the developing retina or in the adrenal medulla. Peripherin is also completely absent from cell bodies in the cerebral and cerebellar cortices. These results indicate that peripherin is found in development only in regions in which it is found in the adult. It can either co-exist with neurofilaments in the same neuron or the two may be independently expressed.
利用免疫荧光染色技术，在小鼠胚胎中研究了III型神经元特异性中间丝蛋白外周蛋白和中间神经丝亚基的表达情况。这两种蛋白最早在胚胎第9天于延髓中被检测到，最初表现为纤维染色，随后在发育中的面神经核和听神经核的细胞体中出现染色。随着胚胎发育，外周蛋白出现向头端和尾端的延伸，在三叉神经节、神经纤维和肠神经系统中也能看到染色。随着脊髓形成，在前角发育中的运动神经元中有抗外周蛋白染色，而中间神经丝亚基在细胞体中的染色较少。两种抗体均对发育中的背根及其进入区进行染色，但外周蛋白存在于二级感觉纤维和连合纤维中，而中间神经丝亚基则不存在。虽然这两种蛋白都存在于背根神经节的神经元中，但其分布有所不同。神经节中较大的外周细胞同时含有这两种蛋白，而较小且更靠近中心的细胞（占神经节细胞的超过60%）仅含有外周蛋白。在交感神经节中也发现了类似的情况，其中有些细胞含有外周蛋白、中间神经丝亚基或两者都有，但大多数神经元的细胞体中仅含有外周蛋白。在发育中的视网膜或肾上腺髓质中未发现外周蛋白。在大脑和小脑皮质的细胞体中也完全没有外周蛋白。这些结果表明，外周蛋白仅在成年期发现其存在的区域中在发育过程中出现。它可以与神经丝在同一神经元中共存，也可能独立表达。
