Poulsen K, Jacobsen J
J Hypertens Suppl. 1984 Dec;2(3):S221-3.
In aggressive male mice with very high plasma renin concentrations the plasma angiotensin II (ANG II) and III (ANG III) concentrations were measured in single mice in order to study whether this renin was active in vivo. The angiotensins were purified and identified by ion-exchange chromatography and High Pressure Liquid Chromatography (HPLC) and quantitated by a specific and sensitive radioimmunoassay. Plasma ANG II and ANG III values were not elevated in aggressive mice compared to normals, in spite of the fact that the plasma renin concentration was increased up to 100 fold and almost equal to the plasma renin-substrate concentration. This indicates an inhibition of the enzymatic renin reaction in vivo during aggression, which is in accordance with the lack of blood pressure increase, and an unchanged pressure sensitivity to ANG II.
为了研究这种肾素在体内是否具有活性，在血浆肾素浓度非常高的攻击性雄性小鼠中，对单只小鼠的血浆血管紧张素II（ANG II）和III（ANG III）浓度进行了测量。通过离子交换色谱法和高压液相色谱法（HPLC）对血管紧张素进行纯化和鉴定，并通过特异性灵敏放射免疫分析法进行定量。尽管血浆肾素浓度增加了高达100倍且几乎与血浆肾素底物浓度相等，但与正常小鼠相比，攻击性小鼠的血浆ANG II和ANG III值并未升高。这表明在攻击过程中体内肾素酶反应受到抑制，这与血压没有升高以及对ANG II的压力敏感性不变是一致的。
