[布宜诺斯艾利斯市污水排放中的小肠结肠炎耶尔森氏菌及相关菌种：使用希曼培养基进行分离]
[Yersinia enterocolitica and related species in the sewage discharge from the city of Buenos Aires: use of Schiemann medium in their isolation].
作者信息
Floccari M E, Peso O A
机构信息
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
出版信息
Rev Argent Microbiol. 1984;16(2):57-66.
Schiemann's medium was used for the isolation of Yersinia enterocolitica and Yersinia enterocolitica-like organisms. These bacteria were isolated in 90% of the samples of sewage of the city of Buenos Aires, both from direct and cold enriched samples. From a total of 19 strains, 8 were classified as Y. enterocolitica (lactose positive), 7 as Y. frederiksenii and 2 as Y. intermedia. Two strains could be included in the latter species (biotypes 4 and 5 from Brenner et al. (14] but a confirmation by DNA hybridization would be useful. All the Y. enterocolitica and Y. frederiksenii were sero and lysotyped according to the Center for Yersinia (Paris, France).
希曼培养基用于分离小肠结肠炎耶尔森菌和类小肠结肠炎耶尔森菌。在布宜诺斯艾利斯市90%的污水样本中都分离出了这些细菌，这些样本既有直接采集的，也有经过冷增菌处理的。在总共19株菌株中，8株被归类为小肠结肠炎耶尔森菌（乳糖阳性），7株为费氏耶尔森菌，2株为中间型耶尔森菌。有两株菌株可归入后一个菌种（布伦纳等人[14]分类的生物型4和5），但通过DNA杂交进行确认会很有帮助。所有的小肠结肠炎耶尔森菌和费氏耶尔森菌都根据法国巴黎耶尔森菌中心的方法进行了血清分型和噬菌体分型。
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