核糖体RNA结构的动力学

Dynamics of ribosomal RNA structure.

作者信息

Erdmann V A, Digweed M, Pieler T

机构信息

Institut für Biochemie, Freie Universität Berlin, Federal Republic of Germany.

出版信息

J Biomol Struct Dyn. 1983 Dec;1(3):657-67. doi: 10.1080/07391102.1983.10507473.

DOI:10.1080/07391102.1983.10507473

Abstract

The structural dynamics of ribosomal 5S RNAs have been investigated by probing single strandedness through enzymatic cleavage and chemical modification. This comparative study includes 5S rRNAs from E. coli, B. stearothermophilus, T. thermophilus, H. cutirubrum, spinach chloroplast, spinach cytomplasm, and Artemia salina. The structural studies support a unique tertiary interaction in eubacterial 5S rRNAs, involving nucleotides around positions 43 and 75. In addition long range structural effects are demonstrated in E. coli 5S rRNA due to the conversion of C to U at position 92.

摘要

通过酶切和化学修饰探测单链性，对核糖体5S RNA的结构动力学进行了研究。这项比较研究包括来自大肠杆菌、嗜热脂肪芽孢杆菌、嗜热栖热菌、深红螺菌、菠菜叶绿体、菠菜细胞质和卤虫的5S rRNA。结构研究支持真细菌5S rRNA中一种独特的三级相互作用，涉及43位和75位附近的核苷酸。此外，由于大肠杆菌5S rRNA中92位的C转换为U，还证明了其长程结构效应。

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