Ames M M, Sanders M E, Tiede W S
Cancer Res. 1983 Feb;43(2):500-4.
Hexamethylmelamine (HMM) is metabolized by rat hepatic microsomal preparations to reactive species which covalently bind to microsomal protein and to calf thymus DNA added to microsomal incubation mixtures. Covalent binding to macromolecules is dependent on the presence of molecular oxygen and reduced nicotinamide adenine dinucleotide phosphate and is catalyzed by cytochrome P-450 monooxygenases. Reduced nicotinamide adenine dinucleotide-dependent covalent binding of [methyl-14C]HMM to microsomal protein is greater than that of [ring-14C]HMM. Reduced nicotinamide adenine dinucleotide phosphate-dependent covalent binding of [ring-14C]HMM and [methyl-14C]HMM to calf thymus DNA added to microsomal incubation mixtures are approximately equal. The [ring-14C]-labeled carbinolamine intermediate in HMM demethylation, N-methylolpentamethylmelamine, covalently binds to microsomal protein and, to a much greater extent, to calf thymus DNA.
六甲基三聚氰胺（HMM）经大鼠肝微粒体制剂代谢生成活性物质，这些活性物质可与微粒体蛋白以及添加到微粒体孵育混合物中的小牛胸腺DNA共价结合。与大分子的共价结合依赖于分子氧和还原型烟酰胺腺嘌呤二核苷酸磷酸的存在，并由细胞色素P - 450单加氧酶催化。[甲基 - 14C]HMM与微粒体蛋白的还原型烟酰胺腺嘌呤二核苷酸依赖性共价结合大于[环 - 14C]HMM。[环 - 14C]HMM和[甲基 - 14C]HMM与添加到微粒体孵育混合物中的小牛胸腺DNA的还原型烟酰胺腺嘌呤二核苷酸磷酸依赖性共价结合大致相等。HMM去甲基化过程中[环 - 14C]标记的氨基甲醇中间体N - 羟甲基五甲基三聚氰胺与微粒体蛋白共价结合，并且在更大程度上与小牛胸腺DNA共价结合。
