Shelly D C, Warner I M, Quarles J M
Clin Chem. 1983 Feb;29(2):290-6.
We describe an indirect detection method for bacterial identification and differentiation, based on selective adsorption of several fluorescent dyes. The lipid, protein, and nucleic acid components of fixed whole cells are stained with two mixtures, each containing two fluorochromes. The unadsorbed dyes were measured simultaneously with a video fluorometer [Clin Chem 22: 1483, 1976]. A dye-absorption matrix of the response can be generated, and we did so for each of nine bacteria. These responses were compared to a control or "complete" response matrix, and the response ratios of each bacterial species for each of the four dyes were calculated and plotted to obtain a characteristic pattern. From the response-ratio plots, plus simple pattern-recognition techniques, we could differentiate among all the bacteria. This rapid, sensitive technique is potentially applicable to a wide variety of bacteria.
我们描述了一种基于几种荧光染料的选择性吸附来进行细菌鉴定和区分的间接检测方法。固定的全细胞的脂质、蛋白质和核酸成分用两种混合物染色，每种混合物包含两种荧光染料。未吸附的染料用视频荧光计同时测量[《临床化学》22: 1483, 1976]。可以生成响应的染料吸收矩阵，我们对九种细菌中的每一种都进行了这样的操作。将这些响应与对照或“完整”响应矩阵进行比较，并计算和绘制每种细菌对四种染料中每种染料的响应比，以获得特征模式。通过响应比图，加上简单的模式识别技术，我们可以区分所有细菌。这种快速、灵敏的技术有可能适用于各种各样的细菌。
