胸腺素α1前体cDNA的合成及通过亲和层析法纯化活性mRNA。
Synthesis of thymosin alpha 1 precursor cDNA and purification of active mRNA by affinity chromatography.
作者信息
Rey-Campos J, Rey-Mendez M, Freire M
出版信息
Int J Biochem. 1983;15(2):155-7. doi: 10.1016/0020-711x(83)90059-9.
PMID:6401638
Abstract
- Thymosin alpha 1 precursor [3H]cDNA-cellulose synthesis was carried out by reverse transcription with RNA-dependent DNA polymerase from avian myeloblastosis virus using oligo(dT)-bound to cellulose as a primer. 2. Unlabelled thymosin alpha 1 precursor cDNA-cellulose was synthetized in a preparative scale to be used in affinity chromatography. 3. Poly(A)-mRNA from calf thymus was subjected to cDNA-cellulose affinity chromatography and an active messenger obtained in a yield of 3% respect to the total thymus poly(A)-mRNA chromatographied. 3. The analysis of the translation products of the purified mRNA have shown it as the thymosin alpha 1 precursor messenger.
摘要
- 胸腺素α1前体[3H]cDNA - 纤维素合成是通过使用与纤维素结合的寡聚（dT）作为引物，由禽成髓细胞瘤病毒的依赖RNA的DNA聚合酶进行逆转录来完成的。2. 未标记的胸腺素α1前体cDNA - 纤维素以制备规模合成，用于亲和色谱。3. 来自小牛胸腺的聚（A）-mRNA进行cDNA - 纤维素亲和色谱分析，获得了一种活性信使，相对于色谱分析的总胸腺聚（A）-mRNA，产率为3%。3. 对纯化mRNA的翻译产物分析表明它是胸腺素α1前体信使。
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