Chojnacki T, Dallner G
J Biol Chem. 1983 Jan 25;258(2):916-22.
The uptake of dietary polyprenols was studied by administering, through a gastric tube, labeled alpha-saturated and alpha-unsaturated polyprenols, with 11 and 19 isoprene residues. The lipids appeared in all organs but mostly in the liver after 16 h where those with 11 isoprenes were in much higher concentration than the prenols with 19 isoprene residues; the distribution in the liver was studied in detail. About 45% of the polyprenols taken up were esterified with fatty acids. A part of the radioactivity (6-30%) appeared in the supernatant but mostly in water-soluble form. Among subcellular fractions, the highest uptake was found in the outer mitochondrial membranes. After 16 h, both 11- and 19-residue alpha-unsaturated injected prenols were present to a large extent as alpha-saturated compounds in liver homogenates and subcellular fractions. About 10-15% of the lipids were phosphorylated. The results suggest that a part of the dolichol phosphate pool, participating in glycosylation reactions, may derive from dietary unsaturated polyprenols which after uptake can be reduced and phosphorylated.
通过胃管给予带有11个和19个异戊二烯残基的标记α-饱和和α-不饱和聚戊烯醇，研究膳食聚戊烯醇的摄取情况。16小时后，脂质出现在所有器官中，但主要存在于肝脏中，其中含有11个异戊二烯的脂质浓度远高于含有19个异戊二烯残基的聚戊烯醇；对肝脏中的分布进行了详细研究。摄取的聚戊烯醇约45%与脂肪酸酯化。一部分放射性（6 - 30%）出现在上清液中，但大多以水溶性形式存在。在亚细胞组分中，线粒体外膜的摄取量最高。16小时后，注入的带有11个和19个残基的α-不饱和聚戊烯醇在肝脏匀浆和亚细胞组分中很大程度上以α-饱和化合物形式存在。约10 - 15%的脂质被磷酸化。结果表明，参与糖基化反应的部分多萜醇磷酸池可能来源于膳食不饱和聚戊烯醇，其摄取后可被还原并磷酸化。
