Cross A S, Zollinger W, Mandrell R, Gemski P, Sadoff J
J Infect Dis. 1983 Jan;147(1):68-76. doi: 10.1093/infdis/147.1.68.
Levels of antibody to the K1 polysaccharide capsule were examined in sera from patients naturally infected with K1-positive Escherichia coli, in sera from volunteers vaccinated with a group B meningococcal vaccine, in immune globulin prepared for intravenous use, and in a preparation of murine IgM monoclonal antibody to group B meningococci. In a phagocytic assay, the monoclonal antibody in mouse ascites fluid killed K1-positive E. coli to a final dilution of 1:250,000 (48 ng of antibody protein/ml); no killing was observed with any of the other antibody sources. This monoclonal antibody, which required human complement and exhibited a prozone when high concentrations of antibody (greater than 6 micrograms/ml) were used, killed all K1-positive strains but none of the K1-negative strains of E. coli tested. Levels of K1-binding antibody in the sera of vaccinated volunteers exceeded antibody levels resulting from natural infection or present in commercially prepared immunoglobulin and were less than those obtained in mouse ascites fluid containing the monoclonal antibody.
对自然感染 K1 阳性大肠杆菌患者的血清、接种 B 群脑膜炎球菌疫苗的志愿者血清、静脉注射用免疫球蛋白制剂以及 B 群脑膜炎球菌鼠 IgM 单克隆抗体制剂中的 K1 多糖荚膜抗体水平进行了检测。在吞噬试验中，小鼠腹水中的单克隆抗体能将 K1 阳性大肠杆菌杀死至终浓度为 1:250,000（48 纳克抗体蛋白/毫升）；其他任何抗体来源均未观察到杀菌作用。这种单克隆抗体需要人补体，并且在使用高浓度抗体（大于 6 微克/毫升）时会出现前带现象，它能杀死所有测试的 K1 阳性大肠杆菌菌株，但对任何 K1 阴性大肠杆菌菌株均无作用。接种疫苗的志愿者血清中 K1 结合抗体水平超过了自然感染产生的抗体水平或市售免疫球蛋白中的抗体水平，但低于含有单克隆抗体的小鼠腹水中的抗体水平。
