Veerman A J, Huismans D R, van Zantwijk C H
Acta Haematol. 1983;69(1):32-5. doi: 10.1159/000206835.
In 83 children with acute lymphoblastic leukaemia (ALL) the immunological phenotype of the lymphoblasts was determined using E rosetting, monoclonal anti-T cell sera, surface immunoglobulin staining and common ALL antiserum. The data were compared with acid alpha-naphthyl acetate esterase (ANAE) and acid phosphatase (AP) cytochemical data. The vast majority of T-ALL cells proved to be ANAE-negative (30/31) and AP-positive (29/31). Null-ALL was always AP-negative (12/12), the ANAE reaction was sometimes positive (4/12). In common ALL, findings ranged from negative to positive for both enzymes. Two cases of B-ALL were ANAE-negative and AP-negative. Enzyme cytochemical determinations gave thus preferential patterns, especially for T-ALL and null-ALL. In common ALL, considerable heterogeneity was found, which may be a reflection of differences in maturation between different cases of common ALL.
在83例急性淋巴细胞白血病（ALL）患儿中，运用E玫瑰花结试验、单克隆抗T细胞血清、表面免疫球蛋白染色及普通ALL抗血清，确定了淋巴母细胞的免疫表型。将这些数据与酸性α-萘乙酸酯酶（ANAE）和酸性磷酸酶（AP）的细胞化学数据进行了比较。绝大多数T-ALL细胞被证明为ANAE阴性（30/31）且AP阳性（29/31）。裸细胞ALL始终为AP阴性（12/12），ANAE反应有时为阳性（4/12）。在普通ALL中，两种酶的结果从阴性到阳性不等。2例B-ALL为ANAE阴性和AP阴性。酶细胞化学测定呈现出偏好性模式，尤其是对于T-ALL和裸细胞ALL。在普通ALL中，发现了相当大的异质性，这可能反映了不同病例的普通ALL在成熟度上的差异。
