Setoguti T, Inoue Y
Cell Tissue Res. 1983;228(2):219-30. doi: 10.1007/BF00204874.
Freeze-fracture images of the parenchymal cells in the parathyroid gland of rats were observed after vitamin D2 plus calcium chloride-suppression and EGTA-activation of secretion. In cells of the suppressed glands, large bulges protruded from the Golgi cisternae, and large granules with a stalk, which are identified as storage granules, suggest that, during maturation, some storage granules may be connected by long tubules with the Golgi cisternae and supplied with secretory products from the Golgi cisternae via these tubules. In the activated glands, presumptive exocytotic and endocytotic specializations of intramembranous particles of the parenchymal cell plasma membrane were frequently observed. In addition, elevations and complementary shallow depressions of various shape and extent were occasionally encountered in the intercellular space. From their morphological characteristics it was concluded that these originated from secretory granule cores, which are discharged from the parenchymal cells into the intercellular space by exocytosis, and it was suggested that discharged granule cores may retain their spherical shape until they fuse to form a flat conglomerate.
在给予大鼠维生素D2加氯化钙抑制及EGTA激活分泌后，观察了甲状旁腺实质细胞的冷冻断裂图像。在受抑制腺体的细胞中，大的凸起从高尔基池突出，带有柄的大颗粒（被鉴定为储存颗粒）表明，在成熟过程中，一些储存颗粒可能通过长小管与高尔基池相连，并通过这些小管从高尔基池获得分泌产物。在激活的腺体中，经常观察到实质细胞质膜内颗粒的推测性胞吐和胞吞特化。此外，在细胞间隙偶尔会遇到各种形状和程度的隆起和互补性浅凹陷。从它们的形态特征可以得出结论，这些起源于分泌颗粒核心，这些核心通过胞吐作用从实质细胞释放到细胞间隙，并且有人提出，释放的颗粒核心在融合形成扁平聚集体之前可能保持其球形。
