Patarroyo M, Biberfeld P, Klein E, Klein G
Cell Immunol. 1983 Jan;75(1):144-53. doi: 10.1016/0008-8749(83)90313-1.
Treatment of human blood mononuclear cells with nanomolar concentrations of phorbol 12,13-dibutyrate (P(Bu)2) induced their aggregation. The phenomenon was seen within a few minutes and reached its maximum manifestation after 20 min. At this time, 20-30% of unfractionated and nylon wool-passed mononuclear leukocytes were in the aggregates. The influence of pretreatment with 2-deoxyglucose, NaN3, EDTA, cyclohexamide, or incubation at 4 degrees C on the phenomenon indicated that it is energy and temperature dependent, requires the presence of extracellular divalent cations, and is independent of protein synthesis. Nonaggregating 2-deoxyglucose-and NaN3-pretreated cells could still bind [3H]P(Bu)2 which rules out the possibility that the phorbol ester molecule acts as a bridge between the aggregated cells. Seventeen percent of the P(Bu)2-treated T-cell population bound untreated autologous and allogeneic cells. The binding property has a certain species specificity because only 4% of the cells interacted with mouse lymphocytes. At the ultrastructural level, the intercellular binding showed broad areas of surface contact (both between lymphocytes and lymphocyte-monocyte) and "trapping" by surface processes was not seen. Aggregated cells did not show cytopathogenic changes.
用纳摩尔浓度的佛波醇12,13 - 二丁酸酯（P（Bu）2）处理人血单核细胞会诱导其聚集。这种现象在几分钟内即可观察到，并在20分钟后达到最大表现程度。此时，20% - 30%未分级的和通过尼龙毛柱的单核白细胞处于聚集体中。用2 - 脱氧葡萄糖、NaN3、EDTA、环己酰胺预处理或在4℃下孵育对该现象的影响表明，它依赖于能量和温度，需要细胞外二价阳离子的存在，且与蛋白质合成无关。未聚集的经2 - 脱氧葡萄糖和NaN3预处理的细胞仍能结合[3H]P（Bu）2，这排除了佛波酯分子作为聚集细胞之间桥梁的可能性。17%经P（Bu）2处理的T细胞群体能结合未经处理的自体和同种异体细胞。这种结合特性具有一定的物种特异性，因为只有4%的细胞与小鼠淋巴细胞相互作用。在超微结构水平上，细胞间结合显示出广泛的表面接触区域（淋巴细胞与淋巴细胞 - 单核细胞之间均有），未观察到表面突起的“捕获”现象。聚集的细胞未显示出细胞病变变化。
