Jacob R A, Gorman N
Clin Chem. 1983 Mar;29(3):564-6.
We describe an automated determination of serum prealbumin (transthyretin), based on the maximum rate of light scatter of an antigen-antibody precipitate. Optimal dilutions of antibody reagent and of 100-microL serum samples are given. Within- and between-assay CVs are less than 5%. Results by this method are nearly identical with those obtained by a radial-immunodiffusion technique. Measuring the rate of light scatter provides a considerably faster test than equilibrium scatter, electrophoretic, or radial-immunodiffusion methods. Treatment before analysis to clear lipemic sera obviates falsely high results.
我们描述了一种基于抗原 - 抗体沉淀物光散射最大速率的血清前白蛋白(转甲状腺素蛋白)自动化测定方法。给出了抗体试剂和100微升血清样本的最佳稀释度。批内和批间变异系数均小于5%。该方法的结果与放射免疫扩散技术获得的结果几乎相同。测量光散射速率比平衡散射、电泳或放射免疫扩散方法提供了更快的检测。分析前对脂血血清进行处理可避免出现错误的高结果。