Belote J M, Baker B S
Dev Biol. 1983 Feb;95(2):512-7. doi: 10.1016/0012-1606(83)90054-4.
The wild-type function of the sex transforming gene transformer-2 (tra-2) is shown to be required for normal spermatogenesis in XY males. A temperature-shift experiment using the tra-2ts2 allele suggests that tra-2+ must function during the middle stages of spermatogenesis to ensure development of functional sperm. Our results, taken together with those of T. Schüpbach (1982, Dev. Biol. 89, 117-127) indicate that the tra-2+ gene functions in the male germ line and thus, in contrast to all other sex determination loci examined to date (doublesex, intersex, transformer), its action is not limited to the soma. Orcein-stained testis preparations from tra-2 males reveal a spermiogenic defect similar to that associated with dominant male sterile (X; autosome) translocations.
性别转换基因transformer-2（tra-2）的野生型功能被证明是XY雄性正常精子发生所必需的。使用tra-2ts2等位基因进行的温度转换实验表明，tra-2+必须在精子发生的中期发挥作用，以确保功能性精子的发育。我们的结果与T. Schüpbach（1982年，《发育生物学》89卷，117 - 127页）的结果共同表明，tra-2+基因在雄性生殖系中发挥作用，因此，与迄今为止检测的所有其他性别决定位点（doublesex、intersex、transformer）不同，其作用不限于体细胞。来自tra-2雄性的orcein染色睾丸制剂显示出与显性雄性不育（X；常染色体）易位相关的精子发生缺陷。
