影响枯草芽孢杆菌D-葡萄糖醇分解代谢相关基因的gut、fruC和pfk突变的染色体定位。
Chromosomal localization of gut, fruC, and pfk mutations affecting genes involved in Bacillus subtilis D-glucitol catabolism.
作者信息
Gay P, Chalumeau H, Steinmetz M
出版信息
J Bacteriol. 1983 Mar;153(3):1133-7. doi: 10.1128/jb.153.3.1133-1137.1983.
Abstract
Mutations affecting the genes involved in B. subtilis D-glucitol catabolism were mapped either by PBS1-mediated transduction or DNA-mediated transformation. It was shown that the genes gutA and gutB coding for the D-glucitol permease and the D-glucitol dehydrogenase, respectively, and regulatory locus gutR are clustered in a gut operon localized between purB and dal close to the pha marker. A mutation affecting fructokinase activity (fruC) was mapped near the gut markers. The fruC gene does not belong to the operon. A mutation affecting phosphofructokinase activity (pfk) was mapped between the leuA and aroG markers.
摘要
通过PBS1介导的转导或DNA介导的转化，对影响枯草芽孢杆菌D-葡萄糖醇分解代谢相关基因的突变进行了定位。结果表明，分别编码D-葡萄糖醇通透酶和D-葡萄糖醇脱氢酶的gutA和gutB基因以及调控位点gutR聚集在一个位于purB和dal之间、靠近pha标记的gut操纵子中。一个影响果糖激酶活性（fruC）的突变位于gut标记附近。fruC基因不属于该操纵子。一个影响磷酸果糖激酶活性（pfk）的突变位于leuA和aroG标记之间。
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