[枯草芽孢杆菌核黄素操纵子额外调控位点的遗传图谱]
[Genetic mapping of an additional regulatory locus of the riboflavin operon of Bacillus subtilis].
作者信息
Kreneva R A, Perumov D A
出版信息
Genetika. 1996 Dec;32(12):1623-8.
Two mutations designated ribR5 and ribR6, which effectively decrease the constitutive expression of the Bacillus subtilis riboflavin operon, were mapped in the 236 degrees region of the B. subtilis genetic map based on linkage analysis with respect to the apt6 marker. Cotransfer indices for the ribR5 and ribR6 markers and apt6 suggest that both ribR mutations are alleles of exactly the same genetic locus. Total results of transductional and transformational crosses indicate the following marker order: pheA-apt6-ribR-azlB-aroD4. Phenotypic peculiarities of the ribR mutants allow us to assume that they belong to an additional subunit of the regulatory protein of the riboflavin operon.
两个名为ribR5和ribR6的突变有效地降低了枯草芽孢杆菌核黄素操纵子的组成型表达，基于与apt6标记的连锁分析，它们被定位在枯草芽孢杆菌遗传图谱的236°区域。ribR5和ribR6标记与apt6的共转移指数表明，这两个ribR突变是完全相同遗传位点的等位基因。转导和转化杂交的总体结果表明标记顺序如下：pheA-apt6-ribR-azlB-aroD4。ribR突变体的表型特征使我们能够假设它们属于核黄素操纵子调节蛋白的一个额外亚基。
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