血小板减少症大鼠血清中存在的血小板生成刺激因子的部分纯化

Partial purification of a thrombocytopoiesis stimulating factor present in the serum of thrombocytopenic rats.

作者信息

Dassin E, Bourebia J, Najean Y, Rosset A M

出版信息

Acta Haematol. 1983;69(4):249-53. doi: 10.1159/000206900.

DOI:10.1159/000206900

Abstract

Successive chromatographic procedures made it possible to isolate thrombocytopoietin from the serum of thrombocytopenic rats. The following steps were taken: DEAE-cellulose phosphate chromatography, Sephadex chromatography, exclusion chromatography on DEAE-Sephadex A-50 gel. The apparent molecular weight of thrombocytopoietin was about 48,000 daltons. Like erythropoietin, thrombocytopoietin is a glycoprotein, its molecular weight is similar. A single form is obtained only when the different purification steps last less than 10 days. Beyond that time, partial degradation may occur.

摘要

连续的色谱分离程序使得从血小板减少症大鼠的血清中分离出血小板生成素成为可能。采取了以下步骤：DEAE - 纤维素磷酸色谱法、葡聚糖凝胶色谱法、DEAE - 葡聚糖凝胶A - 50凝胶排阻色谱法。血小板生成素的表观分子量约为48,000道尔顿。与促红细胞生成素一样，血小板生成素是一种糖蛋白，其分子量相似。只有当不同的纯化步骤持续时间少于10天时才能获得单一形式。超过那个时间，可能会发生部分降解。