Atmeh R F, Shepherd J, Packard C J
Biochim Biophys Acta. 1983 Apr 13;751(2):175-88. doi: 10.1016/0005-2760(83)90172-8.
In this study immunological procedures were used to detect and quantify high-density lipoprotein (HDL) particles of differing apolipoprotein A composition. In the plasma of eight healthy female subjects, 45% of the total apolipoprotein A-I existed in particles (called '(AI)HDL') devoid of apolipoprotein A-II. The remainder circulated in association with apolipoprotein A-II at a molar ratio of approximately 1:1. Nicotinic acid selectively raised the plasma apolipoprotein A-I/A-II ratio by increasing the proportion of (AI)HDL particles. Probucol produced the opposite effect, lowering the plasma concentration of these particles. The kinetic properties of apolipoprotein A-I in total HDL and in the (AI)HDL particle were the same despite the fact that apolipoprotein A-I equilibration between these two species was incomplete. Therefore, there appear to be at least two apolipoprotein A-containing particle populations in HDL which are immunochemically and metabolically distinct.
在本研究中，采用免疫学方法检测和定量不同载脂蛋白A组成的高密度脂蛋白（HDL）颗粒。在8名健康女性受试者的血浆中，总载脂蛋白A-I的45%存在于不含载脂蛋白A-II的颗粒（称为“(AI)HDL”）中。其余部分与载脂蛋白A-II以大约1:1的摩尔比循环。烟酸通过增加(AI)HDL颗粒的比例选择性地提高血浆载脂蛋白A-I/A-II比值。普罗布考产生相反的效果，降低这些颗粒的血浆浓度。尽管这两种载脂蛋白A-I之间的平衡不完全，但总HDL和(AI)HDL颗粒中载脂蛋白A-I的动力学特性是相同的。因此，HDL中似乎至少存在两种含载脂蛋白A的颗粒群体，它们在免疫化学和代谢方面是不同的。
