S'iakste T G, S'iakste N I, Likhtenshteĭn A V
Biokhimiia. 1983 Jan;48(1):136-42.
Treatment of mouse embryo fibroblasts with thio-TEPA (100 micrograms/ml) induced the formation of DNA interstrand cross-links after 10 hours and of DNA one-strand breaks after 20 hours. A short-term incubation of the cells with high concentrations of thio-TEPA (1 mg/ml, 30 min) resulted in a gradual increase of DNA cross-linking, which reached its maximal value after 4 hours of post-incubation. The cross-linking of DNA as well as the formation of DNA one-strand breaks was accompanied by a weakening of DNA-protein interactions in the chromatin. The prolonged existence of DNA and chromatin lesions is supposed to be an important step in the molecular mechanism of action of thio-TEPA.
用硫代替派（100微克/毫升）处理小鼠胚胎成纤维细胞，10小时后诱导形成DNA链间交联，20小时后诱导形成DNA单链断裂。将细胞与高浓度硫代替派（1毫克/毫升，30分钟）进行短期孵育，导致DNA交联逐渐增加，孵育后4小时达到最大值。DNA交联以及DNA单链断裂的形成伴随着染色质中DNA-蛋白质相互作用的减弱。DNA和染色质损伤的长期存在被认为是硫代替派作用分子机制中的一个重要步骤。
