Haldar S, Tiwari R, Bhattacharyya A, Basu A, Chatterjee G C
Folia Microbiol (Praha). 1983;28(1):22-7. doi: 10.1007/BF02877380.
Both nitrofurantoin-sensitive and nitrofurantoin-resistant strains of Vibrio el tor were found to lyze in the presence of Tris-EDTA at alkaline pH. The rate of lysis was appreciably enhanced by lysozyme. The amounts of intracellular components, viz. proteins and carbohydrates, released from the nitrofurantoin-sensitive strain by Tris-EDTA treatment, were significantly lower than those from the nitrofurantoin-resistant strain. Differences in periplasmic proteins released from Tris-EDTA treated cells of nitrofurantoin-resistant and -sensitive strains were revealed by gel electrophoresis.
发现埃尔托弧菌的呋喃妥因敏感株和呋喃妥因耐药株在碱性pH值的Tris-EDTA存在下都会裂解。溶菌酶可显著提高裂解速率。经Tris-EDTA处理后，呋喃妥因敏感株释放的细胞内成分，即蛋白质和碳水化合物的量，明显低于呋喃妥因耐药株。通过凝胶电泳揭示了呋喃妥因耐药株和敏感株经Tris-EDTA处理的细胞释放的周质蛋白的差异。
