用乙二胺四乙酸或溶菌酶裂解产琥珀酸弧菌。
Lysis of Vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme.
作者信息
Wolin M J
出版信息
J Bacteriol. 1966 May;91(5):1781-6. doi: 10.1128/jb.91.5.1781-1786.1966.
Wolin, M. J. (University of Illinois, Urbana). Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bacteriol. 91:1781-1786. 1966.-Cell suspensions of Vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (EDTA) or lysozyme. Lysis occurs at alkaline pH and is prevented by 0.15 m NaCl or KCl or 0.3 m sucrose. The addition of 10(-3)m Mg(++), 10(-3)m spermine, or 10(-2)m Ca(++) prevents lysozyme lysis, and 10(-4)m spermine prevents EDTA lysis. EDTA lysis leads to the formation of a cell ghost, and lysozyme lysis leads to the formation of an empty round body. Freezing and thawing of cells permits lysozyme attack which is not prevented by the protective agents mentioned above. Much of the cell protein, and almost all of the nucleic acids, are released from the cells during EDTA lysis. Treatment of frozen-thawed cells with lysozyme at neutral pH does not cause release of more than 50% of the cell protein and 60% of the nucleic acids of the cells.
沃林，M. J.（伊利诺伊大学厄巴纳分校）。琥珀酸弧菌被乙二胺四乙酸或溶菌酶裂解。《细菌学杂志》91:1781 - 1786。1966年。-琥珀酸弧菌的细胞悬液可被乙二胺四乙酸（EDTA）或溶菌酶裂解。裂解在碱性pH条件下发生，0.15m NaCl或KCl或0.3m蔗糖可阻止其发生。添加10⁻³m Mg²⁺、10⁻³m精胺或10⁻²m Ca²⁺可阻止溶菌酶裂解，10⁻⁴m精胺可阻止EDTA裂解。EDTA裂解导致形成细胞空壳，溶菌酶裂解导致形成空的圆体。细胞的冻融允许溶菌酶攻击，而上述保护剂无法阻止这种攻击。在EDTA裂解过程中，大部分细胞蛋白质以及几乎所有核酸从细胞中释放出来。在中性pH条件下用溶菌酶处理冻融细胞，不会导致超过50%的细胞蛋白质和60%的细胞核酸释放。
Zotero 插件