Basham T Y, Merigan T C
J Immunol. 1983 Apr;130(4):1492-4.
It has been shown that all three classes of interferons enhance the expression of the major histocompatibility class I antigens (HLA-A,B,C;H-2) on a wide variety of cell types (1-10). However, their effect on the expression of the class II antigens (HLA-DR, Ia), which play a major part in cellular interactions that initiate an immune response, is more controversial. The predominate findings have been that the interferons specifically increase the synthesis and expression of only the class I antigens (3, 4, 6, 8, 10, 11). We report here that recombinant interferon-gamma (IFN-gamma) increases the synthesis and expression of the HLA-DR (la-like) antigens as well as beta 2-microglobulin (beta 2-m), a low m.w. subunit of HLA, on human melanoma cells. No increase in HLA-DR was detected on these melanoma cells with leukocyte interferon (IFN-alpha) at doses 400 times higher than the maximum dose of IFN-gamma. These findings were extended to show that pure IFN-gamma also increases the expression of the HLA-DR antigens on normal peripheral blood monocytes, whereas recombinant IFN-alpha at a similar dose had little effect on the expression of this surface antigen. These findings suggest a specialized role for IFN-gamma in immune regulation in comparison with IFN-alpha.
已表明，所有三类干扰素均可增强多种细胞类型上主要组织相容性复合体I类抗原（HLA - A、B、C；H - 2）的表达（1 - 10）。然而，它们对II类抗原（HLA - DR、Ia）表达的影响更具争议性，II类抗原在引发免疫反应的细胞相互作用中起主要作用。主要研究结果表明，干扰素仅特异性增加I类抗原的合成和表达（3、4、6、8、10、11）。我们在此报告，重组干扰素 - γ（IFN - γ）可增加人黑色素瘤细胞上HLA - DR（类Ia）抗原以及β2 - 微球蛋白（β2 - m，HLA的低分子量亚基）的合成和表达。在这些黑色素瘤细胞上，使用比IFN - γ最大剂量高400倍的白细胞干扰素（IFN - α）未检测到HLA - DR增加。这些发现进一步表明，纯IFN - γ也可增加正常外周血单核细胞上HLA - DR抗原的表达，而相似剂量的重组IFN - α对该表面抗原的表达几乎没有影响。与IFN - α相比，这些发现提示IFN - γ在免疫调节中具有特殊作用。
