Hamano T, Asofsky R
J Immunol. 1983 May;130(5):2027-32.
B cell hybridomas with Ia and IgM molecules on the cell membrane were treated with either purified goat anti-mouse mu antibody (anti-mu) or monoclonal rat anti-mouse IgM antibody (anti-IgM). The spontaneous uptake of [3H] thymidine by these cells was markedly inhibited by both reagents. These hybrid cells could be induced to differentiate into IgM-secreting cells in the presence of these reagents at high frequency. Furthermore, the induction of IgM secretion by B cell hybridomas treated with these antibodies was completely T cell independent, and cell division was not required for the differentiative response to anti-mu. In addition, F(ab')2 fragments of anti-mu showed more effects on proliferation and differentiation of these cells than intact anti-mu. Interestingly, TH2.54, a subline of B cell hybridomas, could generate IgG2a production as well as IgM when incubated with anti-mu. These findings suggest very strongly that the interaction of either goat anti-mu or monoclonal rat anti-IgM with surface IgM molecules on the cell membrane of the B cell hybridomas inhibits in vitro spontaneous proliferation, and results in providing signals for differentiation into Ig-secreting cells without T cell factors.
对细胞膜上带有Ia和IgM分子的B细胞杂交瘤，用纯化的山羊抗小鼠μ抗体（抗μ）或单克隆大鼠抗小鼠IgM抗体（抗IgM）进行处理。这两种试剂均显著抑制了这些细胞对[3H]胸苷的自发摄取。在这些试剂存在的情况下，这些杂交细胞能够以高频率被诱导分化为分泌IgM的细胞。此外, 用这些抗体处理的B细胞杂交瘤诱导IgM分泌完全不依赖T细胞，并且对抗μ的分化反应不需要细胞分裂。另外，抗μ的F(ab')2片段对这些细胞增殖和分化的影响比完整的抗μ更大。有趣的是，B细胞杂交瘤的一个亚系TH2.54与抗μ一起孵育时，除了产生IgM外，还能产生IgG2a。这些发现有力地表明，山羊抗μ或单克隆大鼠抗IgM与B细胞杂交瘤细胞膜表面IgM分子的相互作用抑制了体外自发增殖，并在没有T细胞因子的情况下为分化成Ig分泌细胞提供信号。
