Glassy M C, Handley H H, Cleveland P H, Royston I
J Immunol Methods. 1983 Mar 11;58(1-2):119-26. doi: 10.1016/0022-1759(83)90268-5.
A microenzyme-linked immunoassay (EIA) utilizing an immunofiltration manifold has been developed which provides a rapid, simple, and sensitive method of detecting human monoclonal antibody class, concentration, and specificity. In this assay either whole cells or soluble antigens were immobilized on glass fiber filters followed by incubating with the test human hybridoma supernatant with subsequent analysis by EIA. A specially designed 96-chamber immunofiltration plate is employed which serves as both an incubation chamber and as a filtration manifold. The assay described is unique in that small volumes of human hybridoma supernatant are required, crude preparation of only a few target cells are needed, labile cell surface antigens are preserved and it can be completed in 3 h. This assay is well suited for the rapid screening of large numbers of human hybridoma supernatants.
一种利用免疫过滤装置的微酶联免疫分析（EIA）已被开发出来，它提供了一种快速、简单且灵敏的方法来检测人单克隆抗体的类别、浓度和特异性。在该分析中，全细胞或可溶性抗原被固定在玻璃纤维滤膜上，随后与测试的人杂交瘤上清液孵育，接着通过酶联免疫分析进行分析。使用了一种专门设计的96孔免疫过滤板，它既作为孵育室又作为过滤装置。所描述的分析方法的独特之处在于，所需的人杂交瘤上清液体积小，仅需少量靶细胞的粗制品，可保留不稳定的细胞表面抗原，并且能在3小时内完成。该分析非常适合对大量人杂交瘤上清液进行快速筛选。
