Yannopoulos G, Stamatis N, Zacharopoulou A, Pelecanos M
Mutat Res. 1983 Mar;108(1-3):185-202. doi: 10.1016/0027-5107(83)90120-3.
The male recombination second chromosome 23.5 MRF was isolated from the same Greek population (Southern Greece) in which some years ago 31.1 MRF was discovered. A cytological analysis was carried out on the salivary gland chromosomes of the third-instar larvae deriving from crosses of males heterozygous for the 23.5 MRF chromosome II with various laboratory strains, with the following findings. (a) 23.5 MRF caused all types of chromosome rearrangements most of which involved chromosome II. (b) Discernible aberrations were not detected on the X-chromosome or the left arm of chromosome II. (c) The distribution of the break-points involving the second chromosome showed that 23.5 MRF induced breaks at specific sites on this chromosome. (d) The rearrangements induced were not stable; some were lost during the study, and new arrangements were generated. (e) Evidence was provided that 23.5 MRF was able to induce chromosome breakage in mitotic cells of salivary-gland chromosomes as well. (f) A comparison of the present results with data from the same natural population was made, and hypotheses explaining the present results are discussed.
雄性重组第二染色体23.5 MRF是从同一个希腊种群（希腊南部）分离出来的，几年前在该种群中发现了31.1 MRF。对源自携带23.5 MRF第二染色体的杂合雄性与各种实验室品系杂交产生的三龄幼虫的唾液腺染色体进行了细胞学分析，结果如下：(a) 23.5 MRF导致了所有类型的染色体重排，其中大多数涉及第二染色体；(b) 在X染色体或第二染色体的左臂上未检测到明显的畸变；(c) 涉及第二染色体的断点分布表明，23.5 MRF在该染色体的特定位点诱导断裂；(d) 诱导的重排不稳定，有些在研究过程中丢失，并且产生了新的重排；(e) 有证据表明，23.5 MRF也能够在唾液腺染色体的有丝分裂细胞中诱导染色体断裂；(f) 将目前的结果与来自同一自然种群的数据进行了比较，并讨论了解释目前结果的假设。
