Calvi B R, Gelbart W M
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
EMBO J. 1994 Apr 1;13(7):1636-44. doi: 10.1002/j.1460-2075.1994.tb06427.x.
Previous results suggested that the hobo transposable element is active predominantly in the germline of Drosophila. We investigate germline restriction of hobo transposition by testing in vitro modified elements for their ability to mobilize marked elements in vivo. Although intact hobo elements are germline specific, an hsp70 promoter-hobo transposase fusion is active in the soma. Analysis of the hsp70-promoted transcript does not provide evidence for splicing. Moreover, the hobo promoter confers germline bias to a highly sensitive reporter, delta 2-3 P transposase. These results indicate that hobo transposition is germline specific due to regulation of transposase production at the level of transcription. Thus, although hobo is similar to the P transposable element in organization and tissue specificity, it differs in the underlying mechanism governing germline specific activity.
先前的结果表明，hobo转座元件主要在果蝇的生殖系中具有活性。我们通过测试体外修饰元件在体内动员标记元件的能力，来研究hobo转座的生殖系限制。尽管完整的hobo元件具有生殖系特异性，但hsp70启动子-hobo转座酶融合体在体细胞中具有活性。对hsp70启动的转录本的分析未提供剪接的证据。此外，hobo启动子赋予高度敏感的报告基因delta 2-3 P转座酶生殖系偏向性。这些结果表明，由于在转录水平上对转座酶产生的调控，hobo转座具有生殖系特异性。因此，尽管hobo在组织和组织特异性方面与P转座元件相似，但在控制生殖系特异性活性的潜在机制上有所不同。
