Nielsen K H, Rosenbaum B, Stiller J M
Res Vet Sci. 1983 Jan;34(1):68-72.
Optimal reagent parameters for a haemolysis in gel test for detection of bovine anti-Brucella abortus antibody were established. Using an alkali-treated crude lipopolysaccharide antigen attached to J-negative bovine erythrocytes, 0.125 mg of antigen, 0.1 ml of erythrocytes and 0.4 ml of guinea pig complement were required to perform 15 tests with appropriate serum controls. The haemolysis in gel test and an IgM radioimmunoassay (RIA) procedure were compared for their ability to detect the onset of increased serum antibody levels and antibody levels in sera diluted to extinction. While the RIA was more sensitive in amount of antibody detected, the haemolysis in gel test was equally able to detect initiation of antibody synthesis.
确定了用于检测牛抗流产布鲁氏菌抗体的凝胶溶血试验的最佳试剂参数。使用附着于J阴性牛红细胞的碱处理粗脂多糖抗原，进行15次检测并设置适当的血清对照时，需要0.125毫克抗原、0.1毫升红细胞和0.4毫升豚鼠补体。比较了凝胶溶血试验和IgM放射免疫分析（RIA）程序检测血清抗体水平升高的起始情况以及稀释至消失的血清中抗体水平的能力。虽然RIA在检测到的抗体量方面更敏感，但凝胶溶血试验同样能够检测到抗体合成的起始。
