Nielsen K, Cherwonogrodzky J W, Duncan J R, Bundle D R
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
Am J Vet Res. 1989 Jan;50(1):5-9.
Purified O chain of Brucella abortus was passively attached to polystyrene to differentiate antibody responses of cattle vaccinated with B abortus strain 19 from those of naturally infected cattle. In the indirect assay, using O polysaccharide as antigen, a single serum dilution was used and mouse monoclonal antibody to bovine L chain conjugated with horseradish peroxidase was the detection reagent. Measurable antibody was not found in sera of vaccinated cattle, except for 3 sera from cattle that were persistently infected with strain 19. Sera from 25 cattle infected with pathogenic strains contained antibody on the basis of results of indirect enzyme immunoassay, using smooth lipopolysaccharide or O chain as antigens, or results of competitive enzyme immunoassay, using the O-chain antigen. Results in sera from calves with experimentally induced Yersinia enterocolitica serotype 0:9 infection or inoculated with a low dose of B abortus strain 2308 were comparable with those in sera of cattle that were vaccinated with strain 19. The data correlated with those from competitive enzyme immunoassay, using one serum dilution and horseradish peroxidase-conjugated mouse monoclonal antibody to smooth lipopolysaccharide. On the basis of results of the indirect enzyme immunoassay, all sera (except those samples obtained before inoculation) contained antibody to smooth lipopolysaccharide.
将流产布鲁氏菌纯化的O抗原链被动吸附于聚苯乙烯上，以区分接种19号菌株疫苗的牛和自然感染牛的抗体反应。在间接试验中，以O多糖为抗原，使用单一血清稀释度，并以与辣根过氧化物酶偶联的抗牛轻链小鼠单克隆抗体作为检测试剂。除了3份来自持续感染19号菌株的牛的血清外，接种疫苗的牛血清中未检测到可测量的抗体。根据间接酶免疫测定结果（以光滑脂多糖或O抗原链为抗原）或竞争酶免疫测定结果（以O抗原链为抗原），来自25头感染致病菌株的牛的血清中含有抗体。实验性诱导感染小肠结肠炎耶尔森氏菌0:9血清型或接种低剂量2308号流产布鲁氏菌菌株的犊牛血清结果，与接种19号菌株疫苗的牛血清结果相当。这些数据与使用单一血清稀释度和与辣根过氧化物酶偶联的抗光滑脂多糖小鼠单克隆抗体的竞争酶免疫测定结果相关。根据间接酶免疫测定结果，所有血清（接种前采集的样本除外）均含有抗光滑脂多糖抗体。
