Maduagwu E N
Toxicol Lett. 1983 Mar;15(4):335-9. doi: 10.1016/0378-4274(83)90153-4.
The degradation of cyanogenic glycosides was studied in spontaneously fermenting cassava root pulp and in fresh pulp samples pretreated to prevent either endogenous beta-glycosidase activity, fermentation, or both. The rate of disappearance of the glycosides, as measured by hydrocyanic acid (HCN) production in situ, in membrane-sterilised media or in samples containing 1% sodium iodoacetate, was comparable with the untreated control in which 85% of the substrate was broken down within 72 h. Pretreatment of the fresh pulp with the beta-glucosidase inhibitor 1,5-gluconolactone (1%) markedly reduced the rate of disappearance of the cyanogens while inclusion of glucose in this test medium at the 3% level appeared to induce some hydrolysis. Loss of bound (glycosidic) cyanide in sterilised medium containing the glucosidase inhibitor was negligible. The results suggest that the contribution of the fermentation process in cyanide detoxification of pulped cassava roots is minimal.
对木薯根浆自然发酵过程中以及对新鲜浆样进行预处理以抑制内源性β-糖苷酶活性、发酵或同时抑制二者的情况下，生氰糖苷的降解情况进行了研究。通过原位氢氰酸（HCN）生成量、在膜灭菌培养基中或在含有1%碘乙酸钠的样品中测定的糖苷消失速率，与未处理的对照相当，在未处理对照中，85%的底物在72小时内被分解。用β-葡萄糖苷酶抑制剂1,5-葡萄糖酸内酯（1%）对新鲜浆样进行预处理，显著降低了氰化物的消失速率，而在该测试培养基中加入3%水平的葡萄糖似乎会诱导一些水解。在含有葡萄糖苷酶抑制剂的灭菌培养基中，结合（糖苷）氰化物的损失可忽略不计。结果表明，发酵过程对木薯根浆氰化物解毒的贡献极小。
