[通过免疫酶法鉴定铜绿假单胞菌水提取物中的脂多糖]
[Lipopolysaccharide identification in aqueous extracts of Pseudomonas aeruginosa by an immunoenzyme method].
作者信息
Edvabnaia L S, Shakhanina K L, Dubeĭkovskaia Z A, Palkina N A
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1983 Jan(1):36-9.
PMID:6404075
Abstract
To determine the content of lipopolysaccharide (LPS) in P. aeruginosa aqueous extracts the ELISA was used. Membrane filtration and ultracentrifugation followed by precipitation with ammonium sulfate at 80% saturation and gel filtration on Sephadex G-100 have proved to be the most effective methods for purification of the aqueous extract from LPS.
摘要
为了测定铜绿假单胞菌水提物中脂多糖（LPS）的含量，采用了酶联免疫吸附测定（ELISA）法。膜过滤、超速离心，随后用80%饱和度的硫酸铵沉淀以及在葡聚糖G - 100上进行凝胶过滤，已被证明是从LPS中纯化水提物的最有效方法。
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