Jacquez J A
Biochim Biophys Acta. 1983 Jan 19;727(2):367-78. doi: 10.1016/0005-2736(83)90422-4.
Depletion of energy stores of human red cells decreases the maximum transport capacity, Jm, for glucose transport to a value one-third or less of that found in red cells from freshly drawn blood. There is no change in Km. Hemolysis and resealing of red cells with ATP or ADP reverses the decrease in Jm. The maximum effect occurs at concentrations of ATP in the normal range for red cells, however, there is little effect from ADP concentrations in its normal range in freshly drawn red cells. Hemolysis and resealing with ATP gives an increase in Jm and an increase in differential labeling by photolytic labeling with tritiated cytochalasin B. Most of the activation is lost after a second hemolysis-reseal without ATP but about 25% of the activation remains.
人体红细胞能量储备的耗尽会降低葡萄糖转运的最大转运能力Jm，使其降至刚抽取血液中红细胞所发现值的三分之一或更低。Km没有变化。用ATP或ADP对红细胞进行溶血和重新封闭可逆转Jm的降低。最大效应出现在红细胞正常范围内的ATP浓度下，然而，刚抽取的红细胞中ADP浓度在其正常范围内时几乎没有影响。用ATP进行溶血和重新封闭会使Jm增加，并用氚标记的细胞松弛素B进行光解标记时差异标记增加。在没有ATP的情况下进行第二次溶血-重新封闭后，大部分激活作用丧失，但仍有大约25%的激活作用保留。
