Di Minno G, Silver M J, Murphy S
Blood. 1983 Jun;61(6):1081-5.
There have been reports of a 24-48-hr delay in the recovery of platelet cyclooxygenase activity and platelet function after the ingestion of aspirin. However, these studies employed a single aggregating agent to stimulate enzymatic or functional activity. We investigated the effects of some pairs of aggregating agents on 14 platelet-rich plasmas (PRP) from normal subjects 2 and 4 hr after ingestion of 650 mg aspirin and daily up to 72 hr. We studied platelet aggregation and secretion with a lumiaggregometer and thromboxane-B2 formation by radioimmunoassay. Aggregation and secretion occurred as early as 4 hr after aspirin ingestion in response to combinations of arachidonic acid with epinephrine, collagen, or adenosine diphosphate (ADP). Thromboxane formation was detected as early as 4 hr after ingestion of aspirin in response to 1 mM arachidonic acid in combination with 1 microgram/ml collagen. Up to 72 hr, there was a linear return of thromboxane formation stimulated by this combination, reflecting the entry of new platelets into the circulation. In vitro experiments with mixtures of aspirin-free and aspirin-treated platelets showed that the combination of collagen and arachidonic acid (AA) could produce full aggregation and secretion when only 2.5% of aspirin-free platelets were present. Use of the combination of collagen plus AA demonstrates the early entry into the circulation of platelets originating from megakaryocytes whose cyclooxygenase has not been completely acetylated.
有报道称，摄入阿司匹林后，血小板环氧化酶活性和血小板功能的恢复会延迟24至48小时。然而，这些研究采用单一聚集剂来刺激酶活性或功能活性。我们研究了在摄入650毫克阿司匹林后2小时和4小时以及长达72小时内，某些成对的聚集剂对14份来自正常受试者的富血小板血浆（PRP）的影响。我们使用光聚集仪研究血小板聚集和分泌，并通过放射免疫测定法研究血栓素B2的形成。摄入阿司匹林后4小时，花生四烯酸与肾上腺素、胶原蛋白或二磷酸腺苷（ADP）联合使用时，就会出现聚集和分泌。摄入阿司匹林后4小时，当1 mM花生四烯酸与1微克/毫升胶原蛋白联合使用时，就能检测到血栓素的形成。长达72小时内，这种联合刺激的血栓素形成呈线性恢复，反映出新的血小板进入循环。对未用阿司匹林处理的血小板和用阿司匹林处理的血小板混合物进行的体外实验表明，当仅存在2.5%未用阿司匹林处理的血小板时，胶原蛋白和花生四烯酸（AA）的联合使用就能产生完全的聚集和分泌。使用胶原蛋白加AA的联合方法证明，源自巨核细胞的血小板早期进入循环，其环氧化酶尚未完全乙酰化。
