Siess W, Roth P, Weber P C
Thromb Haemost. 1981 Jun 30;45(3):204-7.
Platelets have been implicated in the development of atherosclerotic and thrombotic vascular diseases. Evaluation of platelet aggregation in relation to endogenously formed compounds which affect platelet function may provide information of clinical and pharmacological relevance. We describe a method in which thromboxane B2 (TXB2) formation was analyzed following stimulation of platelet-rich plasma (PRP) with ADP, 1-epinephrine, collagen, and arachidonic acid. In addition, we determined platelet sensitivity to prostacyclin following ADP- and collagen-induced platelet aggregation. The parameters under study were found to depend on the platelet count in PRP, on the type and dose of the aggregating agent used, and on the test time after blood sampling. By standardization of these variables, a reliable method was established which can be used in clinical and pharmacological trials.
血小板与动脉粥样硬化和血栓形成性血管疾病的发生有关。评估血小板聚集与影响血小板功能的内源性化合物的关系，可能会提供具有临床和药理学相关性的信息。我们描述了一种方法，即用二磷酸腺苷（ADP）、肾上腺素、胶原蛋白和花生四烯酸刺激富血小板血浆（PRP）后，分析血栓素B2（TXB2）的形成。此外，我们还测定了ADP和胶原蛋白诱导血小板聚集后血小板对前列环素的敏感性。研究发现，所研究的参数取决于PRP中的血小板计数、所用聚集剂的类型和剂量以及采血后的测试时间。通过对这些变量进行标准化，建立了一种可靠的方法，可用于临床和药理学试验。
