Smith L J, Curtis J E, Messner H A, Senn J S, Furthmayr H, McCulloch E A
Blood. 1983 Jun;61(6):1138-45.
Blast cells from 20 patients with acute leukemia (13 diagnosed myeloblastic and 7 as lymphoblastic, using the FAB classification) were studied using antibodies to lineage-specific differentiation markers. The phenotypic findings were usually consistent with the clinical diagnosis. However, examples were encountered where individual blast cells had a cytoplasmic marker of one lineage and a surface marker of a different lineage (lineage infidelity). Six examples of intramyeloid (two different myeloid lineages in the same cell) and three examples of interlineage infidelity (myeloid and lymphoid markers in the same blast cell) were encountered. No doubly marked cells were found in control material consisting of normal marrow cells, marrow regenerating after transplantation, or multilineage colonies derived from marrow in culture. A significant trend was observed relating the presence of lineage infidelity and failure of remission-induction. The data are interpreted as support for abnormal gene expression in leukemia.
使用针对谱系特异性分化标志物的抗体，对20例急性白血病患者（根据FAB分类，13例诊断为髓母细胞性，7例为淋巴细胞性）的原始细胞进行了研究。表型结果通常与临床诊断一致。然而，也遇到了个别原始细胞具有一个谱系的细胞质标志物和另一个不同谱系的表面标志物的情况（谱系不忠实）。遇到了6例髓内（同一细胞中有两种不同的髓系谱系）和3例谱系间不忠实（同一原始细胞中有髓系和淋巴系标志物）的情况。在由正常骨髓细胞、移植后再生的骨髓或培养的骨髓来源的多谱系集落组成的对照材料中未发现双标记细胞。观察到谱系不忠实的存在与缓解诱导失败之间存在显著趋势。这些数据被解释为支持白血病中异常基因表达。
