Mikulík K, Jiránová A, Janda I, Weiser J
FEBS Lett. 1983 Feb 7;152(1):125-30. doi: 10.1016/0014-5793(83)80496-7.
Ribosomes from cells of Streptomyces aureofaciens producing tetracycline antibiotics (Tc-ribosomes) differ in electrophoretic mobility of ribosomal proteins S2, S10 and L19 from those of the same strain, where the production of tetracyclines was suppressed by changed cultivation conditions (C-ribosomes). Purified tight vacant couples C- and Tc-ribosomes are equally active in the translation of poly(U). Both types of S. aureofaciens ribosomes are more sensitive to tetracycline and chlortetracycline than ribosomes of Escherichia coli in the Phe-tRNA binding and the translation of poly(U).
产生四环素抗生素的金色链霉菌细胞中的核糖体（Tc-核糖体），其核糖体蛋白S2、S10和L19的电泳迁移率与同一菌株在改变培养条件抑制四环素产生时的核糖体（C-核糖体）不同。纯化后的紧密空偶联C-核糖体和Tc-核糖体在多聚尿苷酸的翻译中具有同等活性。在苯丙氨酰-tRNA结合和多聚尿苷酸的翻译方面，这两种类型的金色链霉菌核糖体比大肠杆菌核糖体对四环素和金霉素更敏感。
