兔脾脏中花生四烯酸和前列腺素H2生成血栓素的过程。

Thromboxane formation from arachidonic acid and prostaglandin H2 in rabbit spleen.

作者信息

Hidaka T, Ueta T, Ogura R

出版信息

J Biochem. 1983 Feb;93(2):367-73. doi: 10.1093/oxfordjournals.jbchem.a134189.

DOI:10.1093/oxfordjournals.jbchem.a134189

Abstract

Incubation of [1-14C]arachidonic acid (AA) and [1-14C]prostaglandin (PG)H2 with rabbit spleen homogenate and microsomes resulted in the formation of a substance with the chromatographic properties of thromboxane (Tx)B2. The radiolabeled material was indistinguishable from authentic TxB2 on TLC in three solvent systems and on radiometric gas chromatography. The generation of TxB2-like material from AA and PGH2 was not observed after boiling of the homogenate and microsomes, and was completely inhibited by imidazole (5 mM). The transformation of AA into the TxB2-like material was not observed during incubation in the presence of indomethacin (28 microM). These results indicate that TxB2 is the principal product of arachidonic acid metabolism by the homogenate or microsomes of rabbit spleen.

摘要

将[1-14C]花生四烯酸（AA）和[1-14C]前列腺素（PG）H2与兔脾匀浆和微粒体一起温育，导致形成了一种具有血栓素（Tx）B2色谱特性的物质。在三种溶剂系统的薄层色谱（TLC）和放射性气相色谱上，这种放射性标记物质与 authentic TxB2无法区分。在匀浆和微粒体煮沸后，未观察到从AA和PGH2生成类似TxB2的物质，并且该生成被咪唑（5 mM）完全抑制。在吲哚美辛（28 microM）存在下温育期间，未观察到AA转化为类似TxB2的物质。这些结果表明，TxB2是兔脾匀浆或微粒体花生四烯酸代谢的主要产物。