Toledo-Pereyra L H, Gordon D A, MacKenzie G H
Cryobiology. 1983 Apr;20(2):205-10. doi: 10.1016/0011-2240(83)90008-1.
This study continues our investigations in the area of canine islet cell cryopreservation. Islet cell allografts were frozen using a simple method to -196 degrees C with rapid freezing rates and dimethyl sulfoxide as the cryoprotectant. Good in vitro viability was observed using trypan blue dye exclusion. After intrasplenic transplantation, grafts which did not reject were able to maintain normoglycemia for periods of greater than 60 days. The use of either Cy A as an immunosuppressant or ALG as a graft pretreatment contributed to prolongation of allograft survival in these long-term surviving recipients. These results encourage further studies in this area for potential future clinical application of this technique to human pancreas.
本研究延续了我们在犬胰岛细胞冷冻保存领域的调查。胰岛细胞同种异体移植采用一种简单方法，以快速冷冻速率冷冻至 -196℃，并使用二甲亚砜作为冷冻保护剂。通过台盼蓝染料排斥试验观察到良好的体外活力。脾内移植后，未发生排斥的移植物能够维持血糖正常水平超过60天。使用环孢素A作为免疫抑制剂或抗淋巴细胞球蛋白作为移植物预处理，有助于延长这些长期存活受体中同种异体移植物的存活时间。这些结果鼓励在该领域进行进一步研究，以便将来有可能将该技术临床应用于人类胰腺。
