Whittingham S
J Immunol Methods. 1983 Jun 24;61(1):73-81. doi: 10.1016/0022-1759(83)90010-8.
A solid phase radioimmunoassay is described for the detection of autoantibodies to the saline-soluble extractable nuclear antigens, ribonucleoprotein (RNP) and SS-B (or La). This assay depends on enrichment of antigens from a crude, commercially available (Pel Freez, U.S.A.) extract of rabbit thymus by absorption to the F(ab)2 fraction of specific high titre antibody attached to a microtitre plate. Serum antibody reactive with this antigen is then detected by 125I-labelled Protein A. The assay is simple and is more sensitive than the gel diffusion assays in general use for detecting such antibodies.
本文描述了一种固相放射免疫测定法，用于检测针对盐溶性可提取核抗原、核糖核蛋白（RNP）和SS-B（或La）的自身抗体。该测定法依赖于通过吸附到附着在微量滴定板上的特异性高滴度抗体的F(ab)2片段，从兔胸腺的粗制商业可用提取物（美国Pel Freez公司）中富集抗原。然后通过125I标记的蛋白A检测与该抗原反应的血清抗体。该测定法简单，并且比一般用于检测此类抗体的凝胶扩散测定法更灵敏。
