Tan-Wilson A L, Rightmire B R, Wilson K A
J Immunol Methods. 1983 Jun 24;61(1):99-106. doi: 10.1016/0022-1759(83)90013-3.
Radial immunodiffusion can be used to determine relative antigen-antibody avidities in exactly the same way as demonstrated previously for quantitative immunoelectrophoresis (Birkmeyer et al., 1981). Antigen-antibody interactions of greater avidity result in a greater value of (delta Area/delta [Antigen]) in plots of immunoprecipitin circle area versus antigen concentration while interactions of equal avidity will yield equal values of (delta Area/delta [Antigen]). This was demonstrated using antigens of different weight ranging from 8000 to 66,000.
放射免疫扩散可用于确定相对抗原-抗体亲和力，其方式与之前定量免疫电泳所展示的完全相同（伯克迈尔等人，1981年）。在免疫沉淀环面积对抗抗原浓度的曲线图中，亲和力更强的抗原-抗体相互作用会导致更大的（Δ面积/Δ[抗原]）值，而亲和力相等的相互作用会产生相等的（Δ面积/Δ[抗原]）值。这一点通过使用分子量从8000到66,000不等的不同抗原得到了证明。
