Tan-Wilson A L, Hartl P M, Delfel N E, Wilson K A
Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13901.
Plant Physiol. 1985 Jun;78(2):310-4. doi: 10.1104/pp.78.2.310.
Bowman-Birk soybean trypsin inhibitor (BBSTI) but not Kunitz soybean trypsin inhibitor (KSTI) was found in samples of undifferentiated and partially differentiated Amsoy 71 tissue culture callus. This suggests the differential metabolism of these two classes of proteinase inhibitors, whether the difference be in synthesis, in rates of degradation, or both. The differential metabolism of the proteinase inhibitors is also seen in the plant. Both BBSTI and KSTI were found in the hypocotyl, root, and epicotyl of the Amsoy 71 soybean seedling in addition to their expected presence in the cotyledons. Whereas the ratio of KSTI to BBSTI in the cotyledon was higher, the ratio of BBSTI to KSTI was higher in the extracotyledonary tissues of the seedling. The levels of both classes of proteinase inhibitors declined during seedling growth, except in the epicotyl and the proximal root. In both of these tissues, an increase in BBSTI, but not in KSTI content, expressed as milligrams inhibitor per plant part, occurred.
在未分化和部分分化的Amsoy 71组织培养愈伤组织样本中发现了鲍曼-伯克大豆胰蛋白酶抑制剂（BBSTI），但未发现库尼茨大豆胰蛋白酶抑制剂（KSTI）。这表明这两类蛋白酶抑制剂的代谢存在差异，无论这种差异是在合成方面、降解速率方面，还是两者皆有。蛋白酶抑制剂的这种差异代谢在植株中也可见。除了在子叶中预期存在外，在Amsoy 71大豆幼苗的下胚轴、根和上胚轴中也发现了BBSTI和KSTI。子叶中KSTI与BBSTI的比例较高，而幼苗子叶外组织中BBSTI与KSTI的比例较高。在幼苗生长过程中，两类蛋白酶抑制剂的水平均下降，但上胚轴和近根除外。在这两个组织中，以每株植物部分毫克抑制剂表示，BBSTI含量增加，而KSTI含量未增加。
