Duprey F, Ruskone A, Delpech B, Rambaud J C
Pathol Biol (Paris). 1983 Jan;31(1):60-4.
Immunoglobulin A (IgA) in intestinal secretions is present in several molecular forms among which dimeric IgA linked to the secretory component (S-IgA) predominates. IgA was measured in jejunal perfusates obtained in nine normal subjects by intraluminal perfusion under a proximal occluding balloon of an isotonic and non antigenic solution. The method associated reverse radial immunodiffusion technique or nephelometry, using human serum as standard with chromatography on a polyacrylamide indubiose column. Chromatography separated IgA between two fractions whose elution ratios were the same as those found with mixtures of pure monomeric IgA and S-IgA. A scale was constructed from the latter, allowing the calculation of the percentages of monomeric IgA and S-IgA present in the jejunal perfusates, and that of their total content of IgA.
肠道分泌物中的免疫球蛋白A（IgA）以多种分子形式存在，其中与分泌成分相连的二聚体IgA（S-IgA）占主导地位。通过在近端阻塞球囊下向九名正常受试者的空肠灌注等渗、无抗原溶液，获得空肠灌洗液并对其中的IgA进行检测。该方法将反向放射免疫扩散技术或比浊法与使用人血清作为标准品相结合，并在聚丙烯酰胺靛蓝糖柱上进行色谱分析。色谱法将IgA分离为两个组分，其洗脱比例与纯单体IgA和S-IgA混合物的洗脱比例相同。根据后者构建了一个量表，用于计算空肠灌洗液中单体IgA和S-IgA的百分比及其IgA总含量。
