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黑腹果蝇和哺乳动物中RNA聚合酶II多肽结构基因的鉴定。

Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.

作者信息

Ingles C J, Biggs J, Wong J K, Weeks J R, Greenleaf A L

出版信息

Proc Natl Acad Sci U S A. 1983 Jun;80(11):3396-400. doi: 10.1073/pnas.80.11.3396.

DOI:10.1073/pnas.80.11.3396
PMID:6407013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC394050/
Abstract

Using subclones representing 14 kilobase pairs (kb) of DNA from the Drosophila melanogaster RNA polymerase II (EC 2.7.7.6) X-linked genetic locus, RpII, we have identified four poly(A)+ RNA transcripts in adult flies. The DNA encoding only one of these, a 7-kb transcript, cross-hybridized to mammalian DNA. DNA from alpha-amanitin-resistant (AmaR) Chinese hamster ovary (CHO) and human cells was used to transform the temperature-sensitive (TS) RNA polymerase II Syrian hamster mutant TsAF8. The acquisition of the TS+ AmaR RNA polymerase II phenotype was accompanied by the appearance of donor-DNA-specific restriction fragments that cross-hybridize to the D. melanogaster 7-kb transcript DNA. This D. melanogaster DNA and the related DNA detected in mammalian species must therefore be the structural gene for a RNA polymerase II polypeptide.

摘要

利用代表黑腹果蝇RNA聚合酶II（EC 2.7.7.6）X连锁基因座RpII的14千碱基对（kb）DNA的亚克隆，我们在成年果蝇中鉴定出了四种聚腺苷酸加尾（poly(A)+）RNA转录本。编码其中仅一种转录本（一种7 kb转录本）的DNA与哺乳动物DNA发生交叉杂交。来自α-鹅膏蕈碱抗性（AmaR）中国仓鼠卵巢（CHO）细胞和人类细胞的DNA被用于转化温度敏感（TS）RNA聚合酶II叙利亚仓鼠突变体TsAF8。获得TS + AmaR RNA聚合酶II表型伴随着出现与黑腹果蝇7 kb转录本DNA交叉杂交的供体DNA特异性限制性片段。因此，这种黑腹果蝇DNA以及在哺乳动物物种中检测到的相关DNA必定是RNA聚合酶II多肽的结构基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/be9d34e73062/pnas00637-0264-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/aa48397fcabc/pnas00637-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/6374d75aee8f/pnas00637-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/adb6e37b8951/pnas00637-0263-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/1493edfb68a4/pnas00637-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/be9d34e73062/pnas00637-0264-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/aa48397fcabc/pnas00637-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/6374d75aee8f/pnas00637-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/adb6e37b8951/pnas00637-0263-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/1493edfb68a4/pnas00637-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a5/394050/be9d34e73062/pnas00637-0264-b.jpg

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本文引用的文献

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Mol Cell Biol. 1982 Jun;2(6):666-73. doi: 10.1128/mcb.2.6.666-673.1982.
2
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J Cell Physiol. 1980 Dec;105(3):527-32. doi: 10.1002/jcp.1041050317.
3
Termination of transcription and its regulation in the tryptophan operon of E. coli.
胡萝卜细胞培养物和RNA聚合酶II对鹅膏毒素的敏感性：6'-羟基鹅膏毒素失活的证据。
Plant Physiol. 1985 Feb;77(2):443-9. doi: 10.1104/pp.77.2.443.
4
Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II.克隆和鉴定编码果蝇 RNA 聚合酶 II 的 140kd 亚基的基因。
EMBO J. 1986 Apr;5(4):741-6. doi: 10.1002/j.1460-2075.1986.tb04276.x.
5
A Caenorhabditis elegans RNA polymerase II gene, ama-1 IV, and nearby essential genes.一种秀丽隐杆线虫RNA聚合酶II基因，ama-1 IV，以及附近的必需基因。
Genetics. 1988 Jan;118(1):61-74. doi: 10.1093/genetics/118.1.61.
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Mol Gen Genet. 1993 Nov;241(3-4):327-34. doi: 10.1007/BF00284685.
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Genetics of eukaryotic RNA polymerases I, II, and III.真核生物RNA聚合酶I、II和III的遗传学
Microbiol Rev. 1993 Sep;57(3):703-24. doi: 10.1128/mr.57.3.703-724.1993.
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