Kelso A, Glasebrook A L, Kanagawa O, Brunner K T
J Immunol. 1982 Aug;129(2):550-6.
The production of macrophage-activating factor (MAF) by antigen-stimulated murine T lymphocyte clones has been compared with their cytolytic function and release of other lymphokines. MAF activity was measured by the capacity of peptone-induced peritoneal exudate cells or bone marrow-derived macrophages to lyse 51Cr-labeled tumor cells after incubation with supernatant from the stimulated T cells and a nonactivating, amplifying dose of lipopolysaccharide. Of 72 clones generated against H-2, MIs, H-Y, or Moloney leukemia virus-associated antigens, 68 were found to produce detectable quantities of MAF. Release of MAF by clones 1) occurred within 1 to 12 hr of exposure to antigen, 2) required stimulation with cells of the relevant antigenic specificity, and 3) could also be induced by concanavalin A, indicating that the cloned cells were the source of the activity. The capacity of a clone to produce MAF was independent of its antigenic specificity, cytolytic activity, or ability to produce interleukin 2 or granulocyte-macrophage colony-stimulating activity. In contrast, production of interferon and MAF was not dissociated for any of the clones tested.
已将抗原刺激的小鼠T淋巴细胞克隆产生巨噬细胞激活因子（MAF）的情况与其细胞溶解功能及其他淋巴因子的释放进行了比较。MAF活性通过蛋白胨诱导的腹腔渗出细胞或骨髓来源的巨噬细胞在与刺激T细胞的上清液及非激活、放大剂量的脂多糖孵育后裂解51Cr标记的肿瘤细胞的能力来测定。在针对H-2、MIs、H-Y或莫洛尼白血病病毒相关抗原产生的72个克隆中，发现68个克隆可产生可检测量的MAF。克隆释放MAF的情况如下：1）在接触抗原后1至12小时内发生；2）需要用具有相关抗原特异性的细胞进行刺激；3）也可由伴刀豆球蛋白A诱导，这表明克隆细胞是该活性的来源。一个克隆产生MAF的能力与其抗原特异性、细胞溶解活性或产生白细胞介素2或粒细胞-巨噬细胞集落刺激活性的能力无关。相比之下，在所测试的任何克隆中，干扰素和MAF的产生都没有分离。
