Ito Y, Kira Y, Ebisawa K, Koizumi T, Matsumoto S, Ogata E
Adv Myocardiol. 1983;4:127-35. doi: 10.1007/978-1-4757-4441-5_10.
Synthesis of cardiac structural protein was studied in perfused rabbit hearts using [3H]lysine and perfluorochemical blood substitute. Relative synthesis rate was estimated in adult rabbit heart when both ventricles worked against zero pressure. The decreasing order was troponin complex, actinin complex, myosin, tropomyosin, and actin and was almost the same as that found in an in vivo study. The synthesis rates of myosin B in left and right ventricles were almost equal in hearts without left and right ventricular pressure load. In young rabbit heart with a right ventricular pressure load, an increase in the synthesis rate of right ventricular myosin B was observed along with the concomitant increase in that of left ventricle. As those increases were blocked by neither propranolol nor verapamil, it was suggested that these increases were not mediated by Ca2+ influx or beta-adrenergic receptors.
使用[3H]赖氨酸和全氟化合物血液代用品，在灌注兔心脏中研究了心脏结构蛋白的合成。当两个心室在零压力下工作时，估计成年兔心脏的相对合成速率。其递减顺序为肌钙蛋白复合物、辅肌动蛋白复合物、肌球蛋白、原肌球蛋白和肌动蛋白，这与体内研究中发现的顺序几乎相同。在没有左右心室压力负荷的心脏中，左右心室肌球蛋白B的合成速率几乎相等。在有右心室压力负荷的幼兔心脏中，观察到右心室肌球蛋白B的合成速率增加，同时左心室的合成速率也随之增加。由于普萘洛尔和维拉帕米均未阻断这些增加，提示这些增加不是由Ca2+内流或β-肾上腺素能受体介导的。
