Schweiger H
Res Exp Med (Berl). 1983;182(1):27-32. doi: 10.1007/BF01852284.
Rats were treated with 50 mg phenobarbital (PB) per kg body weight for 7 days prior to or after partial hepatectomy. The activities of aspartate aminotransferase, alanine aminotransferase, and glutamate dehydrogenase were measured in the regenerating liver 1 week following liver amputation. UDP-glucuronyl transferase activity was determined at the time of surgery as well as 7 days later at the time of death. Alanine aminotransferase was induced by PB in rats only treated in the postoperative period, while aspartate aminotransferase and glutamate dehydrogenase were not. The activity of UDP-glucuronyl transferase was increased more than twofold by repeated PB treatment in both normal and regenerating liver. After cessation of therapy, however, the enhanced activities returned to a normal level. It is concluded that UDP-glucuronyl transferase activity in regenerating liver is as inducible as in normal rat liver by repeated PB treatment despite incomplete hepatic regeneration. Preoperative PB treatment alone is not sufficient to stimulate the glucuronylating pathway in the late phase of liver regeneration.
在部分肝切除术前或术后，给大鼠按每千克体重50毫克苯巴比妥（PB）的剂量处理7天。在肝切除术后1周，测定再生肝中天冬氨酸转氨酶、丙氨酸转氨酶和谷氨酸脱氢酶的活性。在手术时以及7天后处死时测定尿苷二磷酸葡萄糖醛酸基转移酶的活性。丙氨酸转氨酶仅在术后接受处理的大鼠中被PB诱导，而天冬氨酸转氨酶和谷氨酸脱氢酶未被诱导。在正常肝和再生肝中，通过重复给予PB处理，尿苷二磷酸葡萄糖醛酸基转移酶的活性增加了两倍多。然而，在停止治疗后，增强的活性恢复到正常水平。得出的结论是，尽管肝再生不完全，但通过重复给予PB处理，再生肝中尿苷二磷酸葡萄糖醛酸基转移酶的活性与正常大鼠肝中一样可被诱导。单独术前给予PB处理不足以在肝再生后期刺激葡萄糖醛酸化途径。
