Nielsen K, Stiller J, Adams G, Williams R
Res Vet Sci. 1983 Mar;34(2):131-7.
Various buffers (0.2 M acetate, pH 4.2; 0.01 M phosphate with 0.15 M sodium chloride, pH 7.2; 0.15 M borate, pH 8.2 and 0.025 M carbonate, pH 9.6) were used as coating buffers with Brucella abortus alkali treated lipopolysaccharide on two types of plastic matrices. Maximum binding occurred using the phosphate buffer. However, as was the case with the other buffers 50 per cent or more of the antigen was removed by five washing procedures. B abortus S19 or S2308, suspended in ammonium acetate-carbonate buffer, pH 8.2, was shown to bind maximally when 10(9) cells were dried onto the plastics. Less than 20 per cent of the cells were removed by five washings.
在两种类型的塑料基质上，使用各种缓冲液（0.2M醋酸盐，pH 4.2；含0.15M氯化钠的0.01M磷酸盐，pH 7.2；0.15M硼酸盐，pH 8.2和0.025M碳酸盐，pH 9.6）作为包被缓冲液，包被经碱处理的流产布鲁氏菌脂多糖。使用磷酸盐缓冲液时结合量最大。然而，与其他缓冲液情况相同，经过五次洗涤程序后，50%或更多的抗原被去除。当将10⁹个细胞干燥在塑料上时，悬浮于pH 8.2的醋酸铵 - 碳酸盐缓冲液中的流产布鲁氏菌S19或S2308显示出最大结合。经过五次洗涤后，少于20%的细胞被去除。
