Nielsen K H, Wright P F, Kelly W A, Cherwonogrodzky J H
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ont., Canada.
Vet Immunol Immunopathol. 1988 Jun;18(4):331-47. doi: 10.1016/0165-2427(88)90160-2.
Enzyme immunoassay has gained wide acceptance for serological diagnosis of bovine brucellosis because of its ability to detect antibody of all isotypes unlike the conventional tests. The indirect enzyme immunoassay, however, presents several parameters that require careful analysis. These parameters include the choice of antigen and antiglobulin-enzyme conjugate reagents for use in the assay, dealing with the large amount of data the semi-automatic or automatic assay can generate and the inter- and intralaboratory standardization and quality control. This review considers the various methods described in the literature and, briefly, how some of the problems have been overcome or how they might be dealt with.
酶免疫测定法因其能够检测所有同种型抗体,与传统检测方法不同,已在牛布鲁氏菌病的血清学诊断中得到广泛认可。然而,间接酶免疫测定法存在几个需要仔细分析的参数。这些参数包括用于该测定的抗原和抗球蛋白 - 酶结合物试剂的选择、处理半自动或自动测定可能产生的大量数据以及实验室间和实验室内的标准化及质量控制。本综述考虑了文献中描述的各种方法,并简要介绍了一些问题是如何被克服的,或者如何处理这些问题。