Hardman J T, Beck M L, Hardman P K, Stout L C
Transfusion. 1983 May-Jun;23(3):233-6. doi: 10.1046/j.1537-2995.1983.23383224902.x.
Red cell samples from a patient who was suffering from massive hemolysis due to a brown recluse spider (Loxosceles reclusa) bite were found to be reactive by indirect antiglobulin test with most ABO-compatible serum samples. Spider venom, enzymes related to those in spider venom, and antisera to plasma proteins and Rh antigens were used to investigate the unusual reactivity of the patient's cells. IgG was detected on the patient's cells by indirect antiglobulin tests. Cells treated with brown recluse spider venom or phosphatidylcholine phosphatidohydrolase reacted similarly. These findings suggest that sphingomyelinase D, which has been identified in brown recluse spider venom, may be related to the unusual reactivity of the patient's cells. Unexpected reactions were observed when venom-treated cells were tested with Rh antibodies: O negative cells absorbed and eluted anti-D from Rh immune globulin; E negative cells were reactive with a commercial anti-E reagent.
一名因被棕色遁蛛（褐皮花蛛）咬伤而遭受大量溶血的患者的红细胞样本，通过间接抗球蛋白试验发现与大多数ABO相容的血清样本呈反应性。使用蜘蛛毒液、与蜘蛛毒液中的酶相关的酶以及针对血浆蛋白和Rh抗原的抗血清来研究患者细胞的异常反应性。通过间接抗球蛋白试验在患者细胞上检测到IgG。用棕色遁蛛毒液或磷脂酰胆碱磷脂水解酶处理的细胞反应相似。这些发现表明，在棕色遁蛛毒液中已鉴定出的鞘磷脂酶D可能与患者细胞的异常反应性有关。当用Rh抗体检测经毒液处理的细胞时，观察到意外反应：O型阴性细胞从Rh免疫球蛋白中吸收并洗脱抗-D；E型阴性细胞与市售抗-E试剂呈反应性。
