Potdevin F, Roncato M, Drupt F, Paris M, Leclerc M
Ann Immunol (Paris). 1983 Jan-Feb;134C(1):105-23.
Immunoelectrophoresis is the usual method for identifying abnormal bands detected by zone electrophoresis. In the last few years, a new technique has appeared, that of immunofixation. This method involves an antibody-antigen precipitation reaction in an electrophoretic strip. The aim of the present study was to compare immunofixation (IFX) and immunoelectrophoresis (IEP) of 101 sera with one or more homogeneous bands upon serum protein electrophoresis: among them 97 bands were subsequently characterized by IFX on agarose gel, and only 50 by IEP. IF appears to be a more sensitive technique for characterizing M-components, especially those with multiple bands. While more rapid than IEP, this new procedure does not seem appropriate for routine use.
免疫电泳是鉴定区带电泳检测到的异常条带的常用方法。在过去几年中，出现了一种新技术，即免疫固定技术。该方法涉及在电泳条带中进行抗体-抗原沉淀反应。本研究的目的是比较101份血清的免疫固定（IFX）和免疫电泳（IEP），这些血清在血清蛋白电泳上有一条或多条均一性条带：其中97条带随后通过琼脂糖凝胶上的IFX进行了特征鉴定，而通过IEP鉴定的只有50条。IF似乎是一种更敏感的技术来鉴定M成分，尤其是那些有多条带的成分。虽然比IEP更快，但这种新方法似乎不适合常规使用。
