Reyes R, Magdaleno V M, Hernández O, Rosado A, Delgado N M
Arch Androl. 1983 May;10(2):155-60. doi: 10.3109/01485018308987557.
Swelling sperm nuclei were assayed, exposing them to the combine action in increasing amounts of seminal plasma 0.1-1.0 ml (124 +/- 21 micrograms of Zn++/ml). An inhibition of almost 30% in the swollen spermatozoa nuclei was observed with 0.1 ml of seminal plasma (12 micrograms of Zn++/ml) reaching to 71% with 74-124 micrograms of Zn++/ml. Inactivated seminal plasma (boiling) induced the same percentage of inhibition (73%) than normal seminal plasma. Dialyzed seminal plasma (31 micrograms of Zn++/ml) produced an inhibition of 88% of swollen spermatozoa nuclei, the same percentage produced with 30-35 micrograms of zinc ions (ZnCl2). Previous release of sperm zinc by preincubation with EDTA 6 mM changed the decondensation kinetics, making sperm nuclei more susceptible to the action of the glycosaminoglycan. No effect was observed in the presence of calcium ions. Therefore, zinc, among its several physiological roles, may act as a nuclear chromatin stabilizer.
对肿胀的精子细胞核进行检测，使其暴露于逐渐增加量的精浆（0.1 - 1.0毫升，锌离子浓度为124 ± 21微克/毫升）的联合作用下。当使用0.1毫升精浆（锌离子浓度为12微克/毫升）时，观察到肿胀的精子细胞核抑制率近30%，而当锌离子浓度为74 - 124微克/毫升时，抑制率达到71%。热灭活的精浆（煮沸）诱导的抑制率（73%）与正常精浆相同。透析后的精浆（锌离子浓度为31微克/毫升）对肿胀的精子细胞核产生88%的抑制率，与30 - 35微克锌离子（氯化锌）产生的抑制率相同。预先用6毫摩尔乙二胺四乙酸（EDTA）孵育使精子锌提前释放，改变了去浓缩动力学，使精子细胞核对糖胺聚糖的作用更敏感。在钙离子存在的情况下未观察到影响。因此，锌在其多种生理作用中，可能充当核染色质稳定剂。
